Features of Sf-RVN cells include: - No Sf-rhabdovirus contamination detectable by a highly sensitive nested RT-PCR assay over more than 100 continuous passages - Growth rates in serum free medium identical to Sf9 Approximately 20 h doubling time in suspension culture in Expression Systems' ESF-921 medium without serum - Recombinant protein productivity in serum free medium identical to Sf9 Tested for three recombinant proteins - Higher baculovirus titers than Sf9 cells 5 to 10-fold higher levels of infectious baculovirus progeny - Better plaque assays than Sf9 cells Sf-RVN cells consistently produce larger, more distinct plaques in traditional plaque assays compared to Sf9 cells |