Sf-RVN is a new, Sf-Rhabdovirus-free S. frugiperda cell line that can seamlessly replace the widely used Sf9 cell line. Features of Sf-RVN cells include: - No Sf-rhabdovirus contamination detectable by a highly sensitive nested RT-PCR assay over more than 100 continuous passages - Growth rates in serum free medium identical to Sf9 Approximately 20 h doubling time in suspension culture in Expression Systems' ESF-921 medium without serum - Recombinant protein productivity in serum free medium identical to Sf9 Tested for three recombinant proteins - Higher baculovirus titers than Sf9 cells 5 to 10-fold higher levels of infectious baculovirus progeny - Better plaque assays than Sf9 cells Sf-RVN cells consistently produce larger, more distinct plaques in traditional plaque assays compared to Sf9 cells How to get Sf-RVN cells: GlycoBac is currently providing Sf-RVN cells to interested parties under commercial Materials Transfer Agreements (for evaluation) and/or licensing agreements (for application) in various fields of use. Please contact Dr. Jarvis directly for additional information. Read more about Sf-RVN cells in our peer-reviewed publication in the journal 'Protein expression and purification' |
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